kramerse at wmvx.lvs.dupont.com responded Re: missing 500 bp band w/ lambda hind3
:I believe you need more mass. If you have 500ng of total mass in the lane
:you will have about 6 ng of mass in the 564bp band. I just looked it up
:in one of my methods books, it says "20 ng per single band is readily
:detectable". Your best bet is to call the supplier of the marker and
:see what they recommend. Hope this helps.
Your sensitivity depends mostly on the intensity and the wavelength
of your UV lightbox. I've got one that can see 1 ng, and one that
would have trouble with 20.
Steve Hardies Hardies at thorin.uthscsa.edu