phenol substitute for DNA/RNA?

at umich.edu
Mon Jul 11 13:32:07 EST 1994


In article <CsoL5r.JFB at crash.cts.com>, rcdana at crash.cts.com (Richard Dana)
wrote:

> Substitute for phenol to do DNA and RNA extractions:
> 
> 	Is there a good substitute?
> 
> 	The widespread use of this reagent should be stopped if someone
> 	can find a safer replacement substance. 
> 
 

If you think about it carefully no reagent which denatures and removes
protein like phenol is ever going to be safe for use. Besides that no one
ever said working in a laboratory was ever going to be safe and free of
injury. Almost any chemical and any protocol contains dangerous and
potenially harmful chemicals that can never be replaced by "safer" ones.
Actually phenol is not nearly as volatile and caustic as many of the other
chemicals used in RNA/DNA extractions.
What about Guanidinium Thiocynate? Beta Mercaptoethanol? Formaldehyde?
Formamide? Even absolute ethanol is not good for you. 


Steven Coon
sdcoon at umich.edu
University of Michigan Medical Center



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