kclark at immsvr.jr2.ox.ac.uk.
Mon Jul 11 08:50:48 EST 1994
In article <2vgu44$ehe at mserv1.dl.ac.uk>
drm21 at mole.bio.cam.ac.uk (David Micklem) writes:
> I used to have a very useful protocol for doing minipreps in 96 well
> plates. Now I can't find the paper, and I can't remember the authors names
> or the journal, nor even the year! I've done a bit of literature searching
> for it, but I just can't find it.
> Does anyone out there know this method and have a reference? An outline of
> the method as I remember it follows:
> Grow up bugs in 1ml tubes on 96well format (available USA/Scientific Plastics).
> Using multichannel pipette, transfer 100ul each to 100ul glycerol for stocks.
> Tranfer another 100ul to another 96-well plate. Pellet cells
> Prep DNA by a slightly modified alkaline lysis procedure. (This is the bit
> that I need the paper for!
> The DNA quality was fine for restriction analysis, but not great for sequencing.
> Thanks for your help.....
If you DNA is in M13 have you tried magnetic bead preps?
I do 96 in about 1 hour using beads from Dynal which have lac Z on
them. These work fine for automatic sequencing.
\/\ /\/\ /\/\ /\/ kclark at immsvr.jr2.ox.ac.uk
\/\ /\/\/\ /\/\/\ /\/ Institute of Molecular Medicine,
\/\/\/ \/\/\/ \/\/\/ Oxford,UK.
\/\/ \/\/ \/\/ I've been here for 5 years and they only
hung me the right way up yesterday.
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