In <Csor2t.KFx at usenet.ucs.indiana.edu> jgraham at bronze.ucs.indiana.edu (the End) writes:
>You may want to try alkaline lysis again, but use ammonium acetate as your
>third solution rather than sodium or potassium acetate*. For cells
>resuspended in 200 ul and denatured with 400 ul of SDS/NaOH, add
>300 ul of 7.5 ammonium acetate at the dissolved pH. This change
>dramatically increase the number of enzymes which cut well in my
>For super-clean sequencing templates, add RNAase to the supernatant
>follwing this step, precipitate with 400 ul of isopropanol, and then
>resuspend the pellet in 2 M ammonium acetate, spin out the "rocks"
>J. E. Graham
>* Morelle, G. Focus 11:1 p.7
>** Lee and Rasheed. 1990. Biotechniques 9(6) p.676
Jim is right. The protocol by Lee & Rasheed is the best.