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Usage of degenerated oligonucleotides

Reinhard Kunze kunze at gen1.genetik.uni-koeln.de
Tue Jul 12 05:36:25 EST 1994

Is there everyone having experience in the use of degenerated oligonucleotides?
My plan is to order a 60 bases long oligonucleotide for site-directed
mutagenesis, which should have four wobble-positions. Could you please send
me your advice, in which ratios 

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