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glutathione fusion protein

Peter Uetz uetz at embl-heidelberg.de
Tue Jul 12 14:49:27 EST 1994


In article <2vgv6a$fdq at mserv1.dl.ac.uk>, mbdxb at s-crim1.dl.ac.uk (D.R. Bell) writes:

> We have a GST fusion protein which comes out as insoluble.
> 
> Has anyone tried solubilising such proteins, and then trying affinity
> purification of the refolded GST-fusion protein?

The following paper describes the solubilization of the protein
from the pellet:


Grieco et al. (1992)
An improved procedure for the purification of protein fused with GST
BioTechniques 13 (6): 856


This paper gives some general troubleshooting advice on insoluble protein:

Smith,D.B. (1993)
Purification of GST fusion proteins
Meth Mol Cell Biol 4: 220



Peter Uetz (UETZ at EMBL-Heidelberg.de)




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