In article <2vgv6a$fdq at mserv1.dl.ac.uk>, mbdxb at s-crim1.dl.ac.uk (D.R. Bell) writes:
> We have a GST fusion protein which comes out as insoluble.
>> Has anyone tried solubilising such proteins, and then trying affinity
> purification of the refolded GST-fusion protein?
The following paper describes the solubilization of the protein
from the pellet:
Grieco et al. (1992)
An improved procedure for the purification of protein fused with GST
BioTechniques 13 (6): 856
This paper gives some general troubleshooting advice on insoluble protein:
Purification of GST fusion proteins
Meth Mol Cell Biol 4: 220
Peter Uetz (UETZ at EMBL-Heidelberg.de)