We have a strange problem with a GST-fusion. We made a few fusion proteins
with the same N-terminus and different, shortened C-termini. Each of those
proteins (the full length included and except the shortest with only a few
aminoacids) seems 15 to 20 kd too large on SDS-page. Of course we verified
the constructs and used pGEX-2T which has 3 stops 3' of the polylinker in
all reading frames.
Any Help ?????
By the way, where can I buy an anti-GST antibody ??
With many Thanks
***** Jean-Marc REICHHART *****
IBMC 15,rue Reni Descartes
Tel. 88 41 70 34 fax 88 60 69 22
reichhart at astorg.u-strasbg.fr