PCR of > 10kb fragments

Parke K. Flick ch994 at cleveland.Freenet.Edu
Wed Jul 13 09:53:50 EST 1994


Dear Reena,
        See the paper by Cheng, et al. PNAS 91, 5695-99 (June, 94).
Also, Perkin-Elmer just introduced their GeneAmp XL kit for
long PCR.  I have not personally tried it, but it is based on the
conditions and reagents described in the above publication.  You can
also contact Dr. Wayne Barnes at Wash. U. Sch. of Med., St. Louis.
His e-mail address is Barnes at biodec.wustl.edu.  He developed
an enzyme system that appears to be effective for amplifications
of up to 40kb or so and is a co-author on the above paper.
Regards,
P. Flick
 
--
Parke K. Flick, Ph.D.  ch994 at cleveland.freenet.edu
USB/Amersham Life Sciences, Inc.
Cleveland, OH  44128  Ph: 216-464-9277; FAX: 216-360-0975

-- 
Parke K. Flick, Ph.D.  ch994 at cleveland.freenet.edu
USB/Amersham Life Sciences, Inc.
Cleveland, OH  44128  Ph: 216-464-9277; FAX: 216-360-0975



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