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DNA from gel and questions

Dongxian Yue dxy at iastate.edu
Thu Jul 14 00:44:03 EST 1994


Dear frinends,

Too many ways to get DNA from agarose gel. Here is another one. 

You dissolve the gel slice in NaI, then mix with Promega Wizard Resin,
follow the Wizard procedure, your DNA will be in H2O in minutes.

So there is no ethanol ppt. 

No elution, no mess up. 

No phenol/chloroform.

Works well for me.

Here is the questions: When agarose is dissolved in NaI, what happened
to the agarose? Is it cleaved or what? 

Is there solvent could dissolve polyacrylamide gel and do not harm RNA?
I need to get small RNA from denature gel, all the methods are so nesty.

A question for Amersham mutangenesis kit users: This kit uses
[alpha]S-dCTP to synthesize the mutant strand. Can this strand survive
in vivo? 

Welcome comments.

Dongxian Yue

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