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Phosphorylate an abasic 5 prime synthetic nucleotide?

Paul Morrison p_morrison at dfci.harvard.edu
Wed Jul 13 20:22:05 EST 1994


Synthetic oligonucleotiders,

 If one made a synthetic oligo and the 5 prime position was made
abasic, could this oligo be phosphorylated? Anyone try this? It seems
DNA kinase would have a good shot at it but might have a hard time
recognising it if there was no base to glom onto ?
Just building a crazy substrate.

Paul Morrison



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