Genomic fly DNA PCR Problems

Peter T. Lin perlin at leland.Stanford.EDU
Thu Jul 14 02:22:39 EST 1994

Does anyone know any good PCR conditions or cycling for a
PCR of D. melanogaster flies wildtype and sco/cyo or any synaptobrevin
mutants?  I'm trying to get the primers to anneal, but they don't
seem to be working.  What are ideal annealing temperatures?  Thanks.

Peter Lin	perlin at


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