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Genomic fly DNA PCR Problems

Peter T. Lin perlin at leland.Stanford.EDU
Thu Jul 14 02:22:39 EST 1994

Does anyone know any good PCR conditions or cycling for a
PCR of D. melanogaster flies wildtype and sco/cyo or any synaptobrevin
mutants?  I'm trying to get the primers to anneal, but they don't
seem to be working.  What are ideal annealing temperatures?  Thanks.

Peter Lin	perlin at leland.stanford.edu


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