question on Wong/McClelland procedure

Daniel Meier dmeier at post.its.mcw.edu
Thu Jul 14 15:24:24 EST 1994


Hello Netters,
  In the methods of Wong and McClelland, PNAS 91:640, the radiolabeling of
the PCR products is done in the presence of 667 micromolar dNTP using 1
microCurie of 32PdCTP.  Does anyone think that the procedure could be
improved using lesser concentrations of dNTPs, or is it possible that
this value is constrained by the requirement for 4 mM MgCl2 (since the two
should be adjusted together)?
  All comments will be appreciated.

Dan Meier
dmeier at post.its.mcw.edu




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