IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

question on Wong/McClelland procedure

Daniel Meier dmeier at post.its.mcw.edu
Thu Jul 14 15:24:24 EST 1994


Hello Netters,
  In the methods of Wong and McClelland, PNAS 91:640, the radiolabeling of
the PCR products is done in the presence of 667 micromolar dNTP using 1
microCurie of 32PdCTP.  Does anyone think that the procedure could be
improved using lesser concentrations of dNTPs, or is it possible that
this value is constrained by the requirement for 4 mM MgCl2 (since the two
should be adjusted together)?
  All comments will be appreciated.

Dan Meier
dmeier at post.its.mcw.edu




More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net