question on Wong/McClelland procedure

[Daniel Meier]

Hello Netters,
  In the methods of Wong and McClelland, PNAS 91:640, the radiolabeling of
the PCR products is done in the presence of 667 micromolar dNTP using 1
microCurie of 32PdCTP.  Does anyone think that the procedure could be
improved using lesser concentrations of dNTPs, or is it possible that
this value is constrained by the requirement for 4 mM MgCl2 (since the two
should be adjusted together)?
  All comments will be appreciated.

Dan Meier
dmeier at post.its.mcw.edu