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RNase-free - HELP

Nancy Lemieux nhl at binkley.cs.mcgill.ca
Fri Jul 15 12:38:58 EST 1994

In article <bbadzio.1124272870B at NEWS.SRV.UALBERTA.CA>,
bbadzio at gpu.srv.ualberta.ca (Bohdana Badzio) wrote:
>  Hello!
>  What is the most reliable - and the least detrimental - anti-RNase
> treatment of 
> microtome or cryostate blades to be used in in situ hybridization?
> How "bad" a blade's baking (eight hours, 200 c) is for its sharpness or its 
> quality in general? Is incubation with hydrogen peroxide (3%) or with DEPC
> (0.1%?) satisfactory? What are other known methods?
>   A bit aside, what kind of RNase inhibitor is commonly used during processing
> tissue sections for ISH; is it used during hybridization step, too?
>   Thanks a lot for reading this and sharing your experience.
>   Bohdana Badzio
>   Department of Biological Sciences, University of Alberta
>   bbadzio at gpu.srv.ualberta.ca
> Bohdana Badzio,
> Department of Zoology, University of Alberta
> wgallin at gpu.srv.ualberta.ca

You might want to dip the blade in chloroform.  This is an alternative to
baking and DEPC and H202.

Nancy Lemieux
Dept. of Biochemistry
McGill University
Montreal, Canada
nhl at binkley.cs.mcgill.ca

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