> Some time back when our lab was trying to amplify ribosomal RNA
>the same problem kept propping up (the presence of bands in the blank
lanes in rapds and in using rRNA gene primers). I think one of our attempt
>was to use DNAase on the polymerase before using it for amplification.
>Is there anyone who does amplify ribosomal RNA who found the right solution.
>I mean the bacterial where contamination from Taq could be problematic?
>bsh at med.pitt.edu
Roche-Applied Biosystems/Perkin Elmer have released a Taq pol which is
guaranteed free of contaminating DNA expressly for bacterial rRNA
Disclaimer: I have nothing to do with any of those companies - I just
saw the rep last week, that's all.
John Nash | Email: nash at nrcbsa.bio.nrc.ca
Institute for Biological Sciences | http://cansnd.cisti.nrc.ca/~nash/home.html
National Research Council of Canada| for an eclectic collection of
All opinions are mine, not NRC's! | WWW molbio and other services.