best way to adjust pH of protein solutions?

Bipin K Dalmia bipin at iastate.edu
Fri Jul 15 07:37:52 EST 1994


what is the best way (quickest) to adjust the pH of a protein solution?
dialysis takes too long and desalting/buffer exchange columns are out
'cause i have 100 mL of solution and it would take a 10-story high
column. directly adding acid/base would cause extreme local pH values
and denature the protein. 

i have to do this prior to running an ion-exchange column so i can't
increase the ionic-strength too much either.

bip
-- 
bipin k. dalmia               the other night i was lying on my bed, looking
bipin at iastate.edu             up at the beautiful stars, and i said to myself, 
n2.bkd at isumvs.iastate.edu     'where the F*CK is my ROOF !!'
--



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