Klenow fragment in Reverse trancription buffer ?

Tracy Aquilla aquilla at salus.med.uvm.edu
Fri Jul 15 13:42:23 EST 1994


In Article <zxiong.56.0005E7F0 at arizvm1.ccit.arizona.edu>,
zxiong at arizvm1.ccit.arizona.edu (Zhongguo Xiong) wrote:
>Dear netter:
>
>I have to set up a reaction in which I need to do a Klenow filling before a 
>reverse transcription reaction. I wonder if any one has carried out Klenow 
>filling in a reverse transcription buffer (50 mM Tris, pH 8.3, 75 mM 
>KCl, 3 mM MgCl2, 20 mM DTT).
>
>Any comments or sugestions are welcome.
>
>
>Xiong
>
>Zxiong at arizvm1.ccit.arizona.edu
    
    Klenow is a rather forgiving enzyme, and this should work. (BUT, I
haven't tried this myself yet!)
                        Tracy



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