Klenow fragment in Reverse trancription buffer ?
aquilla at salus.med.uvm.edu
Fri Jul 15 13:42:23 EST 1994
In Article <zxiong.56.0005E7F0 at arizvm1.ccit.arizona.edu>,
zxiong at arizvm1.ccit.arizona.edu (Zhongguo Xiong) wrote:
>I have to set up a reaction in which I need to do a Klenow filling before a
>reverse transcription reaction. I wonder if any one has carried out Klenow
>filling in a reverse transcription buffer (50 mM Tris, pH 8.3, 75 mM
>KCl, 3 mM MgCl2, 20 mM DTT).
>Any comments or sugestions are welcome.
>Zxiong at arizvm1.ccit.arizona.edu
Klenow is a rather forgiving enzyme, and this should work. (BUT, I
haven't tried this myself yet!)
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