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RNAse Free !?

Aaron Pawlyk aaron at netnews.upenn.edu
Sat Jul 16 23:03:24 EST 1994


When we do slicing for in situ hybridization these are the precautions we

Wipe the blade with EtOH.

Use fresh, ProbeON slides.  Not regular slides.

Wear gloves and a mask.

I don't think you have to woryy too much about RNases on the blade.  After all, 
the tissue you are cutting has RNases in it.  I would just keep the tissue at
the lowest possible temperatures at all times.

			-- Aaron

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