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PCR Primers

Neil Slater njs at med.unc.edu
Sun Jul 17 10:45:58 EST 1994

   I am attempting to PCR a fragment out of a plasmid. The primers I'm
using have large overhangs 5' to the binding region in order to add
restriction sites and sequence that doesn't exist in the plasmid. Does
anyone have any input (ie Thermocycler programing) that will optimize this .

						Thank you,

						Neil Slater

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