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Preparing competent E. coli

Norio Suzuki nosuzuki at wormhole.lab.nig.ac.jp
Tue Jul 12 07:25:55 EST 1994

In article <9407101132591.DLITE.danutabois at delphi.com> danutabois at delphi.com (Danuta Bois) writes:
   I've tried it with DH5alpha and HB101, and the best that I've gotten was
   2x10E6. DH5alpha may have been an old culture (I got it from another lab),

 I think there is no problem for most applications. BUT I heard water is 
qulitical for high competency. Use milli-Q water.
Norio Suzuki
(National Institute of Genetics)
E-mail: nosuzuki at wormhole.lab.nig.ac.jp

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