M13 phage precipitation methods

frandenm at TCPLINK.NREL.GOV frandenm at TCPLINK.NREL.GOV
Mon Jul 18 13:11:11 EST 1994


     Gordon,
     
     PEG8000 is the same as PEG6000 (see Sigma catalog).  The size
     was originally underestimated(?) and listed as 6,000.
     
 
______________________________ Reply Separator _________________________________
Subject: M13 phage precipitation methods
Author:  bssgklm at midge.bath.ac.uk (G K L Munro) at smtp
Date:    7/18/94 11:31 AM


Can anyone explain the reason that some protocols use 30%PEG8000/1.6MNaCl to 
precipitate M13 phage while other protocols use 20%PEG6000/2.5M NaCl?
Also,  what difference does PEG6000 make compared to PEG8000?
     
Gordon Munro
University of Bath
UK
     




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