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Help needed w/ buffer stability

Marvin R McCreary mmccrear at magnus.acs.ohio-state.edu
Mon Jul 18 11:40:38 EST 1994


I am using a 0.05 M Potatssium Phosphate Buffer containing o-dianisidine and
h2o2, as a substrate containing solution for a myeloperoxidase assay.  After 
receiving the solution 10 days ago, their was particulate matter in the 
solution, and the myeloperoxidase activity of a standard solution was decrease 
(presumably due to a bad buffer solution).  The buffer solution had been 
refrigerated as directed.

Does anyone have any suggestions, or experience with these buffers so that I
could avoid this problem?

Thanks in advance.

McCreary.14 at osu.edu



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