I am using a 0.05 M Potatssium Phosphate Buffer containing o-dianisidine and
h2o2, as a substrate containing solution for a myeloperoxidase assay. After
receiving the solution 10 days ago, their was particulate matter in the
solution, and the myeloperoxidase activity of a standard solution was decrease
(presumably due to a bad buffer solution). The buffer solution had been
refrigerated as directed.
Does anyone have any suggestions, or experience with these buffers so that I
could avoid this problem?
Thanks in advance.
McCreary.14 at osu.edu