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Transformation problems with GenecleanII

Dave Bates dobates at ucdavis.edu
Mon Jul 18 11:54:44 EST 1994


Has anyone run into difficulties transforming competent cells after
purification of DNA  with Geneclean? My single cut control (pBK CMV cut
with BamH1) will religate (as seen on a gel) but will not transform
either XL1Blue or HB101 after Genecleaning. 

Dave Bates,                                        dobates at ucdavis.edu
Department of Human Physiology, University of California at Davis
No I don't have a sense of humour, now buy me a beer

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