Protein Concentration Estimation

SCOTT D. gends at leeds.ac.uk
Mon Jul 18 08:25:27 EST 1994


Hia,
I'm trying to estimate the concentration of a protein sample that is 
contaminated with nucleotide (it binds RNA, and the nucleic acid is near 
impossible to get rid of without getting rid of your sample).
Any ideas out there??? Tried Biorad, but I need accuracy better than 5%, and 
obviously Absorbance is a bit tricky with nucleic acid present.
Help much appreciated! 

Dave Scott.
Dept Of Genetics,
University of Leeds.
U.K.
Email: gends%south-01.novell.leeds.ac.uk at gps.leeds.ac.uk



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