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pET expression help needed

Clemens Suter-Crazzolara un691cs at genius.embnet.dkfz-heidelberg.de
Mon Jul 18 05:01:53 EST 1994

hello, dear colleagues,

is it possible to put the t7 tag used in the pET system at THE C-terminus 
of a protein ? is it then still recognized by the corresponding antibody ?
i noticed that novagen offers the HIS TAG both at the N- and C- terminus,
but the T7 tag only at the N-terminus.

thanks, clemens

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