ribosome binding site in eukaryotic promotors
tan at aeolus.vmsmail.ethz.ch
Tue Jul 19 11:30:18 EST 1994
In article <303b26$3fa at amcnix.amc.uva.nl> seppen at amc.uva.nl writes:
>I want to express a eukaryotic gene in E.Coli using the T7 system.
>Can anybody give me some information on different ribosome binding
>sites and the influence on expression?
These two papers might provide the information you seek. Basically says that
ribosome binding site of T7 gene 10 leader enhances translation in E.coli (one
of the reasons why the pET expression system works so well).
Olins and Rangwala, JBC, vol 264, 16973-16976, 1989
Olins et la, Gene, vol 73, 227-235, 1988
Institute for Molecular Biology and Biophysics
ETH-Honggerberg (Swiss Federal Institute of Technology)
8093 Zurich, Switzerland
email: tan at aeolus.vmsmail.ethz.ch
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