Looking for a reference to TEB

[Doug Rhoads]

We have been using for some years now a denaturning gel buffer originally 
described as TEB.  This buffer is 100 mM Tris 10mM Borate 2 mM EDTA (Na 
free).  The buffer has a final pH of 9.2 and allows gels to be run at room 
temp rather than 45-50C.  The problem is I have lost track of the original 
reference to this wonderful buffer.  My recollection is that I got it from 
a Biotechniques article back in '86 or '87.  Unfortunately, our library 
only has issues dating back to 1990.  Is there anyone out there that uses 
this buffer (or developed it) that can turn me on to the reference so that 
I can give dutiful credit where credit is due??

TIA.


Doug Rhoads                  || Dept. of Biological Sciences
drhoads at mercury.uark.edu     || 601 Science Engineering
drhoads at uafsysb.uark.edu     || University of Arkansas
501-575-3251                 || Fayetteville, AR 72701