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lymphocyte transfection

Martin Kennedy mkennedy at chmeds.ac.nz
Tue Jul 19 20:30:16 EST 1994

In article <3047hb$8cg at news.u.washington.edu>, rgrant at u.washington.edu (Richard Grant) writes:
> We are attempting to transfect plasmid DNA into lymphocytes in culture 
> and would like to know if anyone has any ideas on how to increase the 
> efficiency.  If you know of a protocol or could give me a reference for 
> lymphocyte transfection I would really appreciate it.  Thanks.
> Rgrant at u.washington.edu

Try the following sources of information:

T-lymphocytes			BIORAD Bulletin #1349
Primary human lymphocytes	BIORAD Bulletin #1348

Boehringer Mannheim, in a recent newsletter, claimed that DOTAP mediated 
transfection of human lymphoid cells is farily efficient.  Could be worth 
scoring a sample to try out.



NNNN   NN  Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz)  ZZZZZZZ  
NN NN  NN       Cytogenetic and Molecular Oncology Unit          ZZZ
NN  NN NN           Christchurch School of Medicine            ZZZ
NN   NNNN              Christchurch, New Zealand              ZZZZZZZ
	      Phone (64-3)364-0880  Fax (64-3)364-0750

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