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PCR vs. DNA Fingerprinting?

M. L. Huang rin0mxw at bumed30.med.navy.mil
Wed Jul 20 11:06:44 EST 1994

On Thu, 14 Jul 1994 20:49:45 CDT, 
U27111 at uicvm.uic.edu  <U27111 at uicvm.uic.edu> wrote:

>I just read the Associated Press article on the DNA fingerprinting
>done on the glove found in the O.J. Simpson case.
>They make a distinction between DNA fingerprinting and PCR and this
>confused me.  I thought all DNA testing was as a result of PCR,
>otherwise, how do they have enough of a sample to work with?
>As a matter of fact, the way the article described it, it almost
>sounded like a southern blot.
>Any comments or explanations would be very helpful.
>Thanks in advance.

I think the process is like this:
     first, PCR to get enough DNA
     cut with specific enzymes
     Southern blot
     look for unique/signature bands, thus polymorphism or fingerprints

Both are used in forensic testing. PCR alone cannot give any 
definite answers. Even both may not always be conclusive either.

************************************ ......................................
Mark L. Huang, Ph.D.                         __l__   ______    __/\___/^\ 
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E-mail: rin0mxw at bumed30.med.navy.mil              Zaijian!                                                      
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Opinions stated here are solely mine and are not those of the U. S. Navy.

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