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Mini-Prep Blues

Benoit_Hebert at IAF.UQUEBEC.CA Benoit_Hebert at IAF.UQUEBEC.CA
Mon Jul 18 21:01:24 EST 1994


gc (genecutl at mendel.berkeley.edu) wrote:

> I've been using a boiling-lysis protocol for mini-preps and it used
> to work beautifully. 
...
> The problem is now the mini-prep gods no longer smile on me and I'm
> lucky to get a single sample to work out of twenty.  What can have gone
> wrong?  Please don't tell me I have to go back to phenol/chloroform
> hell.

there was an article in Biotechniques a few months ago regarding optimization 
of the boiling prep. You might want to take a look. (sorry, but I left the 
last 10 issues at the lab...)

Benoit Hebert

========================================
Benoit_Hebert at iaf.uquebec.ca

Armand-Frappier Institute
Virology Research Center
Laval, Quebec, Canada    H7N 4Z3
phone: (514) 687-5010, ext. 4636
fax: (514) 686-5626
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