373A sequencing of GC-rich templates

Steve Marsh smarsh at portia.caltech.edu
Wed Jul 20 13:10:08 EST 1994


In article <1994Jul6.115932.24398 at oxvaxd>, kclark at immsvr.jr2.ox.ac.uk.
(Kevin Clark) wrote:
> 
> We are having problems sequencing through a region of approx 200 bases
> of almost total G's and C's. Does anyone have any experience of this
> kind of template and how to get through it.

ABI also recomends increasing the Mg++ concentration in the mixture. I will
check on the concentration and let you know. The other solution is to use
sequenase to run  your reactions. This is not supposed to have any problems
with GC rich regions. 

Steve Marsh smarsh at portia.caltech.edu



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