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PAGE gels cracking

R.G. Walters mbrgw at s-crim1.dl.ac.uk
Thu Jul 21 07:28:27 EST 1994


In article <dotzlaw-200794174921 at murphy.biochem.umanitoba.ca>, dotzlaw at ccu.umanitoba.ca (Helmut Dotzlaw) says:
>
>A quick question - is there a way to prevent our 12.5% SDS mini-gels from
>cracking when drying under vacuum?

If you can't increase the strength of the vacuum,
reducing the percentage of bisacrylamide helps prevent cracking.


Robin Walters.                      Robert Hill Institute, Sheffield UK.

A fact is an opinion that everyone agrees with.



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