In article <30k6g4$3r1 at news.iastate.edu>, bipin at iastate.edu (Bipin K
[comments about purifying protein and wanting to store for long time deleted]
> 50 mM tris-hcl pH 8.0
> 125 mM nacl
> 0.5 mM PMSF
> 0.5 mM EDTA
> 1 mM DTT
> 0.05 % NaN3
> 1 % triton x-100
> 50 % glycerol
> protein concentration= 1.1 mg/ml
> any suggestions/comments?
Storage buffer looks fine. For long term storage, I would suggest
aliquoting into Eppendorf tubes, flash freezing in liquid nitrogen and
storing at -80C. For shorter term (i.e. weeks), you may find it
convenient to have stuff at -20C. Since you've got 50% glycerol in the
storage buffer, you can simply take out what you need without having to
thaw the sample.
Hope this helps.
Institute for Molecular Biology and Biophysics
ETH-Honggerberg (Swiss Federal Institute of Technology)
8093 Zurich, Switzerland
email: tan at aeolus.vmsmail.ethz.ch