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PAGE gels cracking

Kevin M. Carr carrkevi at student.msu.edu
Thu Jul 21 10:40:31 EST 1994


In article <1994Jul21.112021.29153 at news.unige.ch>, menoud at cmu.unige.ch
(Pierre-Alain Menoud) wrote:

> In article <dotzlaw-200794174921 at murphy.biochem.umanitoba.ca>,
dotzlaw at ccu.umanitoba.ca (Helmut Dotzlaw) says:
> >
> >Hi all,
> >
> >A quick question - is there a way to prevent our 12.5% SDS mini-gels from
> >cracking when drying under vacuum?
> >
>  
> The best trick, I found, to PA gels from cracking is to incubate them in
5-10% glycerol in water after destaining. 
> 
> 

Helmut,

I used to use the glycerol trick (for 10% gels) to keep them from curling
and it works great *if* you seal them in an airtight container after they
are dried (seal-a-meal bags e.g.).  If left exposed to the atmosphere,
over time they would reabsorb moisture and end up a shattered mess. (I
finally decided curled up gels were better than dealing with this
problem.)

Hope this helps.



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