tips on casting sequencing gel

pzheng at VAXA.WEEG.UIOWA.EDU pzheng at VAXA.WEEG.UIOWA.EDU
Thu Jul 21 15:53:53 EST 1994


If you are still casting your own sequncing gels like me, here is a tip for 
you. Put sigmacote on both plates[despite what you have read from sequencing 
protocols]and proceed as usual. I started this as a mistake, but it worked 
beautifully everytime. This way you don't have to worry about air bubbles and
the gel will always stick to the bottom plate when you take them apart.
john 
pzheng at vaxa.weeg.uiowa.edu



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