anti-CAT antibody in westerns

Richa Behari richa at LELAND.STANFORD.EDU
Thu Jul 21 13:38:15 EST 1994

		Has anybody experienced that CAT (Chloramphenicol acyl
transferase) assays work in enzymatic detection and the anti-CAT antibodies
work beautifully on the same sample in IFA (Indirect immunofluorescent
assays) but refuse to work on western blots of the same samples?
Has anybody tried anti-CAT antibodies from 5pime-3prime or Boehringer
(or is there any other source?) in Westerns or Immunoprecipitations and
got them to work? All ideas, protocols experiences would be most welcome.
Richa at leland.stanford.edu

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net