RNA isolation

Anthony C. acc4636 at tamsun.tamu.edu
Thu Jul 21 12:59:46 EST 1994


In article <1994Jul15.182505.16139 at alw.nih.gov>,
Jim Owens  <jow at helix.nih.gov> wrote:
>2	Add up to 100 mg of tissue to 2.0 ml of RNase-ALL at room temperature
About how many cells is this if Im using cell culture instead of cells directly
from tissue?  The molecular biology book says 1ml denaturing solution 
(4M guanidium) per 10^7 cells, a guy down the hall says his kit uses
1 ml per 2X10^6 cells (about 5 times as much).  thanks!




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