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pET expression help needed

Clemens Suter-Crazzolara un691cs at genius.embnet.dkfz-heidelberg.de
Fri Jul 22 07:47:19 EST 1994


> 
> In your posting you write:
> >
> >hello, dear colleagues,
> >
> >is it possible to put the t7 tag used in the pET system at THE C-terminus 
> >of a protein ? is it then still recognized by the corresponding antibody ?
> >i noticed that novagen offers the HIS TAG both at the N- and C- terminus,
> >but the T7 tag only at the N-terminus.
> >
> >thanks, clemens
> >    
> >
> 
> T7 isn't a tag. It's a promoter element recognized by T7 RNA polymerase
> which 
> is essential for transcription. 
> 
> 




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