>> In your posting you write:
> >hello, dear colleagues,
> >is it possible to put the t7 tag used in the pET system at THE C-terminus
> >of a protein ? is it then still recognized by the corresponding antibody ?
> >i noticed that novagen offers the HIS TAG both at the N- and C- terminus,
> >but the T7 tag only at the N-terminus.
> >thanks, clemens
>> T7 isn't a tag. It's a promoter element recognized by T7 RNA polymerase
> is essential for transcription.