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long range PCR

Garry P. Nolan phactor at netcom.com
Fri Jul 22 20:23:40 EST 1994

Mwatson at uniwa.uwa.edu.au wrote:
: Dear People of the world can anybody give me more info on long-
: range PCR.  I have heard it is possible to PCR 20kb or more and
: I am keen to find out how.

In my lab we have done long range PCR by simply adding a 1:64 dilution of 
vent polymerase into Taq + Taq buffer.  We then do the PCR normally.  We 
easily get 10 kB.  We also always have 5%DMSO in all our reactions.  It 
worked great.

Garry P. Nolan
Molecular Pharmacology
Stanford University

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