Mwatson at uniwa.uwa.edu.au wrote:
: Dear People of the world can anybody give me more info on long-
: range PCR. I have heard it is possible to PCR 20kb or more and
: I am keen to find out how.
In my lab we have done long range PCR by simply adding a 1:64 dilution of
vent polymerase into Taq + Taq buffer. We then do the PCR normally. We
easily get 10 kB. We also always have 5%DMSO in all our reactions. It
Garry P. Nolan