Gel purification of RNA
bhandary at wccf.mit.edu
Fri Jul 22 20:40:00 EST 1994
In article <grandoni.774910351 at soma>, grandoni at soma.UMDNJ.EDU (Jerry Grandoni) writes...
>Does anyone know of a good method for purification of in vitro-synthesized
>RNA from a polyacrylamide gel?
> Jerry Grandoni, Ph.D. Tel. 609-566-6047
> Dept. of Molecular Biology Fax. 609-566-6195
> Univ. of Med. and Dent. of NJ
> 2 Medical Center Dr.
> Stratford, NJ 08084-1489
I excise the band, and soak it overnight at 37oC in 0.1M NaAc, 10mM EDTA, pH
6.0, with 1% phenol. The supernatant is removed, and 2.5 volumes of ethanol
is added. Dry ice 10'; 15,000 x g 15min. A single rinse with 80% ethanol
removes an traces of phenol remaining. I've found this better than using SDS
as a nuclease inhibitor; the SDS is more difficult to remove.
c/o bhandary at wccf.mit.edu
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