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Protein Concentration Methods

ANDY PHILLIPS andy.phillips at afrc.ac.uk
Fri Jul 22 03:02:46 EST 1994

Any method for protein estimation that depends on dye binding is likely to be 
pretty inaccurate, unless you use a standard curve from the same protein as 
your unknown. This is because the amino acid sequence of the protein 
significantly affects the amount of dye bound - as mentioned by an earlier 
posting, some proteins don't stain at all.  The same, of course, is true of 
OD280. I guess the best estimate of absolute protein concentration would be 
from amino acid analysis.

A second point: any assay is only as good as the standard curve. And what 
method was used to assay the standard protein??


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