Protein Concentration Methods

ANDY PHILLIPS andy.phillips at afrc.ac.uk
Fri Jul 22 03:02:46 EST 1994


Any method for protein estimation that depends on dye binding is likely to be 
pretty inaccurate, unless you use a standard curve from the same protein as 
your unknown. This is because the amino acid sequence of the protein 
significantly affects the amount of dye bound - as mentioned by an earlier 
posting, some proteins don't stain at all.  The same, of course, is true of 
OD280. I guess the best estimate of absolute protein concentration would be 
from amino acid analysis.

A second point: any assay is only as good as the standard curve. And what 
method was used to assay the standard protein??

Andy 

 -----------------------------------------------------------------------------
 RFC-822 : ANDY.PHILLIPS at BBSRC.AC.UK            : University of Bristol
 X400    : G=ANDY;S=PHILLIPS;O=BBSRC;P=UK;C=GB  : Long Ashton Research Station 
 Phone   : +44-275-392181 ext:257               : Long Ashton
 Fax     : +44-275-394281                       : Bristol, BS18 9AF, UK
 -----------------------------------------------------------------------------



More information about the Methods mailing list