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Mutagenesis with Sodium bisulfite

John Brunstein brunstei at UNIXG.UBC.CA
Fri Jul 22 11:53:39 EST 1994


	Why not just linearize with ClaI, blunt-end the site, and re-ligate?


On Thu, 21 Jul 1994, Duncan Clark wrote:

> Hi Folks,
> 
> I want to knock out a unique Cla I site in a kan gene in vector. I do not
> care what it becomes providing I lose it. Has anyone used sodium 
> bisulfite for similar purposes and if so have they a protocol etc.
> 
> Many thanks
> 
> Duncan 
> 
> -----------------------------------------------------------------------------
> Duncan Clark                        | Internet:    duncan at genesys.demon.co.uk
> GeneSys Ltd.                        | Compuserve:  100015.1406 at compuserve.com
> -----------------------------------------------------------------------------
> 
> 
> 



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