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Plasmid Prep Scale-up

Roger Graham flo at unixg.ubc.ca
Fri Jul 22 10:30:02 EST 1994


When doing large scale plasmid preps using alkaline lysis (e.g. 50 g E.coli
wet weight) what is minimum volume of reagents required to sustain maximum
efficiency of plasmid recovery ? I'm sure scaling up directly from miniprep
amounts (1.5 ml culture pellet = 100 ul solnI, 200ul solnII, 150ul solnIII
of Birnboim and Doly procedure) would be overkill.

Thanx for your opinions.

Roger Graham
Dept. of Biochemistry
University of British Columbia
Vancouver, B.C., Canada
email : flo at unixg.ubc.ca

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