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Plasmid Prep Scale-up

Roger Graham flo at unixg.ubc.ca
Fri Jul 22 10:30:02 EST 1994


Netters,

When doing large scale plasmid preps using alkaline lysis (e.g. 50 g E.coli
wet weight) what is minimum volume of reagents required to sustain maximum
efficiency of plasmid recovery ? I'm sure scaling up directly from miniprep
amounts (1.5 ml culture pellet = 100 ul solnI, 200ul solnII, 150ul solnIII
of Birnboim and Doly procedure) would be overkill.

Thanx for your opinions.


-- 
Roger Graham
Dept. of Biochemistry
University of British Columbia
Vancouver, B.C., Canada
email : flo at unixg.ubc.ca



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