Kan vs Amp for eukaryotic vectors ?

Roger Graham flo at unixg.ubc.ca
Fri Jul 22 10:35:58 EST 1994


Netters,

Pardon my ignorace, but is there any reason that E.coli - mammalian cell
shuttle vectors for transfection are always based on ampicillin selection
in E. coli? Is there some complication with expression/sequences of
kanamycin or tetracycline or ... genes in mammalian host cell if shuttle
plasmid is resistant to other antibiotics ? Or is this just because
historically they are all derived from pUC ? Are there kanamycin resistance
based mammalian transfection vectors around ?

 Thanx,

-- 
Roger Graham
Dept. of Biochemistry
University of British Columbia
Vancouver, B.C., Canada
email : flo at unixg.ubc.ca



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