Oligo degradation trepidation
mkennedy at chmeds.ac.nz
Sun Jul 24 00:58:30 EST 1994
In article <Ct8zz7.BJG at ucdavis.edu>, szcooley at chip.ucdavis.edu () writes:
....Entire subject matter of previous 2 posts removed....!
> the problem. Operon (a company in California) claim that primers go bad
> at -20 oC. Currently I am storing mine at -70 oC or lypholizing aliquots.
> Mike Cooley mbcooley at ucdavis.ucdavis.edu
Is this for real???? Anybody got any hard evidence? Do I worry about my
-20 degree stored oligos (which probably amount to more than a years worth of
my salary)! Does anyone have a good reason why oligos might "go off" at -20,
but not at -70 degrees? Is anyone form Operon out there...?
Appreciate any comments,
NNNN NN Martin A Kennedy (E-mail = mkennedy at chmeds.ac.nz) ZZZZZZZ
NN NN NN Cytogenetic and Molecular Oncology Unit ZZZ
NN NN NN Christchurch School of Medicine ZZZ
NN NNNN Christchurch, New Zealand ZZZZZZZ
Phone (64-3)364-0880 Fax (64-3)364-0750
More information about the Methods