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lymphocyte transfection

ALASTAIR MACKAY amackay at welchlink.welch.jhu.edu
Sun Jul 24 17:13:30 EST 1994


I have had success in electroporating HeLa and other cells, with 
expression of cDNAs ranging from 2% (bad day) to 70% (good day).  
Adherent cells are trypsinized then electroporated in suspension, so I 
don't see why this wouldn't work with lymphocytes.  Current Protocols has 
a discussion on the subject.  Conditions might approximate 300 Volts at 
960 uF in a 4 mm cuvette.

Cheers, Alastair Mackay




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