In article <Dress-220794113146 at chuck.biosci.arizona.edu> Dress at biosci.arizona.edu (Virginia Dress) writes:
>Subject: Re: Protein Concentration Estimation
>From: Dress at biosci.arizona.edu (Virginia Dress)
>Date: 22 Jul 1994 18:18:48 GMT
>How about figuring out the extinction coefficient for your protein? I
>disagree that most protein assays are crap.
However, IMO most protein assays are grossly abused!
>Any protein assay is only going to give you
>a truly accurate estimate of your protein if you have That protein purified
>to use as the standard in your assay.
I'd go further - a truly accurate estimate of your protein is only possible
if you purify it before measuring (and then you have to contend with
losses during purification). At the very least, it is safest to remove
all non-protein material before the assay, or at least check that it
doesn't give a reading! Standard assays tel you about problematic lab.
reagents, but not about non-protein natural products which could be in your
Jonathan B. Marder '
Department of Agricultural Botany | Internet: MARDER at agri.huji.ac.il
The Hebrew University of Jerusalem | /\/ Bitnet: MARDER at HUJIAGRI
Faculty of Agriculture |/ \ Phone: (08 or +9728) 481918