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band-shift assays

daniel schott dhs3 at postoffice.cornell.mail.edu
Mon Jul 25 08:53:41 EST 1994

Does anyone have expertise in band-shift (protein-Dna binding) assays?  A
friend of mine is having horrible trouble repeating a series of
experiments,  her samples get stuck in the wells.  Due to the nature of the
experiment, she has to use very low concentrations of DNA and no
non-specific DNA competitor.  She doesn't think the problem is aggregation.
 Is the purity of the acrylimide absolutely vital for this stuff to work? 
Reply to bd14 at cornell.edu, and I'll pass on suggestions.  Thanks!  

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