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DNA synthesizers

John E. Fox altabios at bham.ac.uk
Mon Jul 25 07:35:39 EST 1994


In article <1994Jul23.105853.30027 at cobra.uni.edu>, jurgenson at cobra.uni.edu says:
>
>I am currently looking at oligo nucleotide synthesizers.  I have used the
>Beckman Oligo 1000.  I will soon be trying out the chruachem P250.  I have
>considered the new one made by Pharmacea,  and perhaps will try out one from
>ABI.  I also have the oportunity to purchase a used ABI 380 ?  Does any one
>have any insite on the used machine from ABI? or any of the other models listed
>above?  Is their a machine that I should look at that is not listed here.  My
>synthesis needs are for teaching and research .  In molecular biology the oligo
>synthesis rate may be considerable for short periods.  Any advice would be most
>welcome.  My impression is that the Beckman is easy to use and convenient.  It
>has a built in trytl monitor,  but its seems to use a lot of solvent  and the
>reagents they sell are twice the price of those from other suppliers.  


Try the Millipore 'Expedite'. Its very fast, 17bases/hour on two oligos and is very cheap to run.
I have one and eventually would buy another. Solvent use is about 4ml per base.



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