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Potyvirus Purification

Steve Wylie wylie at CSUVAX1.MURDOCH.EDU.AU
Mon Jul 25 20:41:57 EST 1994

Has anyone found an efficient method for purifying potyviruses from plant 
tissue? I have used the PEG 8000 pption/CsCl gradient method of Hammond 
and Lawson (1988) which results in very clean particles but low yield. 
There are several methods out there but with limited plant material and 
time one can not try them all! Any comments appreciated,
Steve Wylie, Murdoch University, Western Australia

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