a week ago, I posted a tip on how to cast a better sequencing gel with all
my good intensions. However, it looks like there are people having big
trouble casting gels like what I described. I apologized if you followed my tipsand got into trouble. Maybe you are using a different coating chemical or a
different apparatus, I have casted more than 15 gels using sigmacote on both plates without having problem pouring acrylamide solution into the space. But do
proceed with caution.
pzheng at vaxa.weeg.uiowa.edu